cloningandexpression相关论文
Cloning and expression profiling of endoglucanase gene in the Chinese mitten crab (Eriocheir sinensi
In this study,a endoglucanase gene was cloned from the Chinese mitten crab (Eriocheir sinensis) by the rapid amplificati......
Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the
The moderately halophilic bacterium Halomonas sp.QHL1, isolated from Qinghai Lake in China, was identified as a member o......
通过ＤＮＡ体外重组技术，以ｐＥＴ－３ｂ为表达载体，构建了重组表达质粒ｐＥＴ－６Ｒ（Ｂ）和ＰＥＴ－６Ｒ（Ｂ）４，分别编码２８ｋＤ和ｈＩＬ－６Ｒ配基结合区片段及其５３ｋＤ的二联体蛋白，并为酶切分析和ＤＮＡ序列分析所证实，ＳＤＳ－ＰＡＧＥ分析......
AIM: To amplify HBV-RNase H gene fragment and expression of RNase H for further use in the studies of HBV associated liv......
本文以多聚酶链反应扩增得到的ＬＴＢ基因ＤＮＡ片段为探针，克隆了含猪源大肠杆菌ＬＴＢ基因的７７５ｂｐＨｉｎｄⅢ酶切片段，对插入片段进行了核苷酸序列测定：将含ＬＴＢ基因的ＥｃｏｒⅠ......
